Whatman™ 10485374 Protran™ BA85 Blotting Sandwich With 0.45µm Nitrocellulose Membrane, Dimensions: 7 x 8.5cm (Pack of 20)

This Protran™ BA85 blotting sandwich is made of a precut nitrocellulose membrane and two sheets of 3MM Chr blotting paper. Prepackaged into a sandwich to help save time. Protran™ nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran™ membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as ‘nitrocellulose’ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran™ membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent. Unlike PVDF membranes, Protran™ nitrocellulose does not require a methanol prewetting step. This makes Protran™ the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary. In addition to high binding capacity, Protran™ nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions. The 0.45µm pore size membrane (BA85) is suitable for larger molecular weight samples. A key benefit of the proprietary Protran™ nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran™. Protran™ nitrocellulose membrane binding capacity: 80 to 150µg/cm2. Autoclavable (liquid cool cycle). Membrane: Protran™ BA85 Nitrocellulose. Pore Size: 0.45µm. Dimensions: 7 x 8.5cm. Whatman™ #: 10485374.

This Protran™ BA85 blotting sandwich is made of a precut nitrocellulose membrane and two sheets of 3MM Chr blotting paper. Prepackaged into a sandwich to help save time. Protran™ nitrocellulose (NC) membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran™ membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as ‘nitrocellulose’ may actually contain large amounts of cellulose acetate which will lower the protein binding capacity. Protran™ membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent. Unlike PVDF membranes, Protran™ nitrocellulose does not require a methanol prewetting step. This makes Protran™ the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary. In addition to high binding capacity, Protran™ nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions. The 0.45µm pore size membrane (BA85) is suitable for larger molecular weight samples. A key benefit of the proprietary Protran™ nitrocellulose formula is the proven shelf life of bound proteins. Empirical evidence shows that proteins maintain molecular recognition activity for five years on Protran™. Protran™ nitrocellulose membrane binding capacity: 80 to 150µg/cm2. Autoclavable (liquid cool cycle). Membrane: Protran™ BA85 Nitrocellulose. Pore Size: 0.45µm. Dimensions: 7 x 8.5cm. Whatman™ #: 10485374.