Cytiva's Whatman™ 28-9031-32 SpinTrap™ Protein A High Performance (HP), Sample Volume: 600?L, Pack Size: 16 Columns
- Binding Capacity/Column: > 1mg human IgG well
- For use with Standard microcentrifuge
- Storage: 2-30°C, 20% Ethanol, Wetted Part: No
- Application: Enrichment of target proteins Purification of antibodies, Centrifugation
- Resin Type: Sepharose High Performance
- Sold Individually
- Manufacturer Part No: WHA-28-9031-32
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Details
Protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain Protein A Sepharose HP For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provide high yield. Fast binding kinetics and high capacity provide high yield. Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. Prepacked* with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap Protein A HP prepacked columns. Easy scale-up with HiTrap Protein A HP prepacked columns. Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Documents & Videos
Protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain Protein A Sepharose HP For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provide high yield. Fast binding kinetics and high capacity provide high yield. Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. Prepacked* with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap Protein A HP prepacked columns. Easy scale-up with HiTrap Protein A HP prepacked columns. Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required. There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.