Cytiva's Whatman™ 28-9546-53 PGEX-2T, 25µg, Selectable Marker (s): Plasmid Confers Resistance to 100?g/ml Ampicillin

  • Recognition Site: Thrombin
  • Order Information: Order E. coli BL21 separately
  • A tac promoter for chemically inducible, high-level expression ofgST-tagged recombinant proteins
  • Very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity
  • PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product
  • Sold Individually
  • Manufacturer Part No: WHA-28-9546-53
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Item Number: WHA-28-9546-53

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Details

Simplified package with only the pGEX vector in the pack (E. coli BL21 will continue to be available separately, code no 27-1542-01. Updated Product Specification sheets. Maintained product quality and quantity. New code numbers. A tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins. An internal lacIq gene for use in any E. coli host. Very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity. PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product. Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease, (see PreScission Protease) between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site

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Simplified package with only the pGEX vector in the pack (E. coli BL21 will continue to be available separately, code no 27-1542-01. Updated Product Specification sheets. Maintained product quality and quantity. New code numbers. A tac promoter for chemically inducible, high-level expression of GST-tagged recombinant proteins. An internal lacIq gene for use in any E. coli host. Very mild elution conditions for release of fusion proteins from the affinity matrix, thus minimizing effects on antigenicity and functional activity. PreScission Protease, Thrombin, or Factor Xa recognition sites for cleaving the desired protein from the fusion product. Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease, (see PreScission Protease) between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site

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