Glass open-ended cloning cylinders can be used to clone a single cell or group of cells. The ends of the cloning cylinder are typically dipped into sterile silicone grease before pressing to the bottom of a culture dish to create an isolated well. Glass cell cluture cylinders are fully autoclavable or can be dry heat sterilized for sterile cell culture and tissue culture work. Reusable.
PYREX® open ended disinfectant testing cylinders are designed for use in the Disinfectant Testing Procedure as described in the 1992 J.A.O.A.C. Hard Surface Carrier Test for Efficacy Testing of Disinfectants: Collaborative Study. Tube is glazed on both open ends. Fully autoclavable for sterile applications. Reusable. Material: Borosilicate Glass Code 7740.
These sterile cloning cylinders are designed specifically for clone isolation. A small amount of grease is smeared on the tapered end of the cylinder and the cylinder is placed on the clone chosen for segregation. A small aliquot of Trypsin or EDTA is added to the cylinder, and after a brief incubation at 37° C the cells lose attachment. After harvesting these detached cells within the cylinder they can be moved to another vessel for propagation.
These sterile paper cloning discs are designed for high throughput labs which clone large numbers of cells. They are ideal for cell transfer and propagation. Cloning discs reduce the risk of leaks, contamination and drying out when compared to using standard cloning cylinders. Sterile paper discs permit transport to 24-well culture plates. Sterilized by gamma irradiation, lot stamped for accurate tracing, and shipped with a Certificate of Sterilization.