Boekel® 240200 RapidFISH (Fluorescent In-Situ Hybridization) Microscope Slide Hybridizer, Temperature Range: Ambient +5° to 100°C, 120V

The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures for FFPE (formalin fixed paraffin embedded tissue) samples, cell suspensions, and other tissue preparation procedures. Temperature control and humidity are essential for FISH probes to bind to RNA and DNA. The RapidFISH has a self-clamping (included) slide tray design that maintains the required humidity. Microscope slides in the RapidFISH do not require coverslips to be glued because the removable tray of the slide incubator traps moisture in the system. Elimination of this step decreases processing time and ensures that the specimens stay hydrated during processing. The system has an easy to use control system for controlling temperature, time and controlled temperature steps. The device has a small footprint (WxDxH: 11 x 15.96 x 8.09-inch) and still maintains excellent temperature stability and uniformity because of its dual heater convection system. FISH testing is used worldwide to identify biological markers in the form of DNA/RNA.

Application Overview 

Molecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of: Prenatal & Cancer diagnosis, Molecular cytogenetic of birth defects and mental retardation, Chromosomal abnormalities, Clinical genetics, Neuroscience, Reproductive medicine, Toxicology, Microbial ecology, Evolutionary biology, Comparative genomics & Cellular genomics, Differentiation between benign and malignant tumors

The majority of this 700 million market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined.

The equipment necessary is: pH meter, balance, stir plate, micro-centrifuge, refrigerator, freezer
Tissue preparation will require: microtome, water bath, oven
Performing the FISH procedure will require the following equipment and materials in addition to reagents: slide dryer, water baths, hybridization oven, Coplin jars, coverslips, staining dishes, rubber cement, fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography

Boekel Scientific #: 240200.

The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures for FFPE (formalin fixed paraffin embedded tissue) samples, cell suspensions, and other tissue preparation procedures. Temperature control and humidity are essential for FISH probes to bind to RNA and DNA. The RapidFISH has a self-clamping (included) slide tray design that maintains the required humidity. Microscope slides in the RapidFISH do not require coverslips to be glued because the removable tray of the slide incubator traps moisture in the system. Elimination of this step decreases processing time and ensures that the specimens stay hydrated during processing. The system has an easy to use control system for controlling temperature, time and controlled temperature steps. The device has a small footprint (WxDxH: 11 x 15.96 x 8.09-inch) and still maintains excellent temperature stability and uniformity because of its dual heater convection system. FISH testing is used worldwide to identify biological markers in the form of DNA/RNA.

Application Overview 

Molecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of: Prenatal & Cancer diagnosis, Molecular cytogenetic of birth defects and mental retardation, Chromosomal abnormalities, Clinical genetics, Neuroscience, Reproductive medicine, Toxicology, Microbial ecology, Evolutionary biology, Comparative genomics & Cellular genomics, Differentiation between benign and malignant tumors

The majority of this 700 million market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined.

The equipment necessary is: pH meter, balance, stir plate, micro-centrifuge, refrigerator, freezer
Tissue preparation will require: microtome, water bath, oven
Performing the FISH procedure will require the following equipment and materials in addition to reagents: slide dryer, water baths, hybridization oven, Coplin jars, coverslips, staining dishes, rubber cement, fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography

Boekel Scientific #: 240200.