This 11 rxn size NovaBlue Singles™ Competent Cells is designed for ultimate convenience and reliability in high-efficiency plasmid transformation of E. coli in less than 8 minutes. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes eliminating the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance). Material: Singles Competent Cells. Use: High-Efficiency Plasmid Transformation Of E. coli. Storage: Below -70°C. Ship: Dry Ice Only. Size: 11 rxn. EMD Chemicals® #: 70181-3.