Sigma T6666-50G Trizma® Hydrochloride Solution, 0.5 Molar, BioXtra Grade, 50g Bottle

Sigma-Aldrich T6666-50G
Item Number:SIG-T6666-50G
Manufacturer Part No:T6666-50G
Manufacturer:Sigma-Aldrich
Units:50g Bottle

Details

  • pH 3.5-5.0 (0.5 Molar in Water), ≥99.0% (Titration)
  • Synonyms: TRIS HCl; TriS Hydrochloride; Tris(hydroxymethyl)aminomethane Hydrochloride
  • CAS Number 1185-53-1
  • 50g Bottle
This Trizma® Hydrochloride (Tris HCl), BioXtra Grade is a high-purity biological buffer component supplied as a 0.5 M solution, designed for precise pH control in molecular biology, biochemistry, and cell culture applications. Identified by CAS 1185-53-1, Trizma HCl is widely used in the preparation of buffer systems within the physiological pH range (approximately pH 7–9), making it essential for maintaining stability in sensitive biological processes. Tris-based buffers are foundational in laboratory workflows, particularly in buffer preparation for electrophoresis, protein analysis, and nucleic acid research. Trizma HCl is commonly combined with Tris base to create buffering systems such as TAE and TBE, which are extensively used in gel electrophoresis for DNA and RNA separation. This reagent is also utilized in sample preparation, staining procedures, protein purification, and immunoassays, supporting consistent and reproducible results across research and diagnostic environments. Its high solubility and reliable buffering capacity make it a trusted choice for laboratories requiring accuracy in pH-dependent reactions.

Documents & Videos

This Trizma® Hydrochloride (Tris HCl), BioXtra Grade is a high-purity biological buffer component supplied as a 0.5 M solution, designed for precise pH control in molecular biology, biochemistry, and cell culture applications. Identified by CAS 1185-53-1, Trizma HCl is widely used in the preparation of buffer systems within the physiological pH range (approximately pH 7–9), making it essential for maintaining stability in sensitive biological processes. Tris-based buffers are foundational in laboratory workflows, particularly in buffer preparation for electrophoresis, protein analysis, and nucleic acid research. Trizma HCl is commonly combined with Tris base to create buffering systems such as TAE and TBE, which are extensively used in gel electrophoresis for DNA and RNA separation. This reagent is also utilized in sample preparation, staining procedures, protein purification, and immunoassays, supporting consistent and reproducible results across research and diagnostic environments. Its high solubility and reliable buffering capacity make it a trusted choice for laboratories requiring accuracy in pH-dependent reactions.