Cytiva's Whatman™ 17-1014-01 Q Sepharose High Performance Anion Exchange Chromatography Media, Ligand: Quaternary Amine, Pack Size: 75mL
- BioProcess resin: Yes, Ion Exchanger Type: Strong anion exchanger
- Matrix: 6% cross-linked agarose, Ionic Capacity: 0.14-0.20mmol Cl-/ml
- Storage: 4 to 30°C, 20% Ethanol
- Particle size, D50v: ~34µm, Particle Size: 24-44µm
- pH stability, operational: 2–12, pH stability, CIP: 1–14
- Sold Individually
- Manufacturer Part No: WHA-17-1014-01
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Details
Q Sepharose High Performance is a strong anion exchange BioProcess resin with high resolution designed for high intermediate and polishing steps in downstream processing Strong anion exchanger for intermediate and final polishing steps High resolution, high capacity separation with high recovery High chemical stability for effective CIP/sanitization BioProcess resin supported for industrial applications and well-established in approved processes The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. Q Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. Q Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide Ligand Coupling Method: Ether linkages Flow Rate: > 5.0 ml/min Pressure/Flow Specification: 150 cm/h (at < 3 bar, 25°C, 10 cm bed height) Flow velocity: 30-150cm/h Exclusion limit (Mr) (globular proteins): ~4 × 10⁶ Chemical stability: Stable in commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH, 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS Charged Groups: - N+(CH₃)₃ Certificate of Analysis: Yes
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Q Sepharose High Performance is a strong anion exchange BioProcess resin with high resolution designed for high intermediate and polishing steps in downstream processing Strong anion exchanger for intermediate and final polishing steps High resolution, high capacity separation with high recovery High chemical stability for effective CIP/sanitization BioProcess resin supported for industrial applications and well-established in approved processes The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool. Q Sepharose High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups. It is designed for intermediate purification and polishing applications and to be used when resolution and capacity have priority. Q Sepharose High Performance has high chemical stability and withstands effective CIP/sanitization protocols using sodium hydroxide Ligand Coupling Method: Ether linkages Flow Rate: > 5.0 ml/min Pressure/Flow Specification: 150 cm/h (at < 3 bar, 25°C, 10 cm bed height) Flow velocity: 30-150cm/h Exclusion limit (Mr) (globular proteins): ~4 × 10⁶ Chemical stability: Stable in commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH, 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS Charged Groups: - N+(CH₃)₃ Certificate of Analysis: Yes