Cytiva's Whatman™ 28-9042-65 illustra™ Blood Genomic Prep Mini Spin Kit, Packsize: 250, Binding Capacity: 60µg

  • Application: Used for Several Downstream Applications Including Real-time PCR, Endpoint PCR, Multiplex PCR, and Restriction Enzyme Digests
  • Sample Volume Min: 50µL Whole Blood, Buffy Coat, Bone Marrow Cells, 2µL Nucleated Blood†, 300µL Whole Blood‡
  • Sample Volume Max: 300µL Whole Blood, Buffy Coat, Bone Marrow Cells, 10µL Nucleated Blood†, 1000µL Whole Blood‡
  • Yield: 4-6µg/200µL Whole Blood, Elution Volume Max: 200µL,Purity (A260/A280) Min: 1.7
  • Purity (A260/A280) Max: 1.9, Total Time of Preparation: < 20min, Protocol Length: < 5min
  • Sold Individually
  • Manufacturer Part No: WHA-28-9042-65
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Item Number: WHA-28-9042-65

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illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells. For the rapid and reproducible isolation of high-quality genomic DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells. High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing. Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse. Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests. The purification process involves minimal shearing resulting in the production of 5 to 10μg of good quality, intact genomic DNA from a 200μL sample. Input sample volumes can vary from 50μL to 1mL. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card. Kit Contents: Proteinase K, lyophilized powder: 2 vial (2 × 60mg), Lysis buffer type 10: 2 × 125mL, Wash buffer type 6: 30mL (Add 120mL Absolute Ethanol before use), Elution buffer type 5: 60mL, illustra blood mini column: 5 × 50, Collection tubes: 5 × 50

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illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells. For the rapid and reproducible isolation of high-quality genomic DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells. High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing. Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse. Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests. The purification process involves minimal shearing resulting in the production of 5 to 10μg of good quality, intact genomic DNA from a 200μL sample. Input sample volumes can vary from 50μL to 1mL. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card. Kit Contents: Proteinase K, lyophilized powder: 2 vial (2 × 60mg), Lysis buffer type 10: 2 × 125mL, Wash buffer type 6: 30mL (Add 120mL Absolute Ethanol before use), Elution buffer type 5: 60mL, illustra blood mini column: 5 × 50, Collection tubes: 5 × 50

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