Cytiva's Whatman™ 28-9042-67 illustra™ Plasmid Prep Midi Flow Kit, Complete Packsize:25, Capacity: ? 250?g
- Application: Used For the Rapid Extraction and Purification of High-Quality Plasmid DNA from Medium Scale
- Sample Volume Min.: 25mL Culture, Sample Volume Max.: 150mL Culture
- Purity (A260/A280) Min.: 1.8, Purity (A260/A280) Max.: 2.5
- Total Time of Preparation: Approx. 215min, Protocol Length: Approx. 40min
- Capacity: ≥ 250μg
- Sold Individually
- Manufacturer Part No: WHA-28-9042-67
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Details
illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume. illustra plasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 ml for high copy number plasmid DNA and up to 500 ml for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA. The kit contains fast-flow plasmid 250 columns, resuspension, lysis, neutralization, wash, elution, and TE buffers, and a full protocol booklet with a detachable, quick reference protocol card. Application: Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification Kit Contents: Lysis buffer type 11: 2 × 360 ml, Lysis buffer type 12: 2 × 360 ml, Lysis buffer type 13: 2 × 360 ml, Wash buffer type 2: 5 × 480 ml, Elution buffer type 3: 480 ml, TE buffer type 1: 120 ml, illustra Fast-Flow plasmid 250 columns: 2 × 50, Column stand: 1, Waste reservoir: 1, Adapters: 12
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illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume. illustra plasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 ml for high copy number plasmid DNA and up to 500 ml for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA. The kit contains fast-flow plasmid 250 columns, resuspension, lysis, neutralization, wash, elution, and TE buffers, and a full protocol booklet with a detachable, quick reference protocol card. Application: Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification Kit Contents: Lysis buffer type 11: 2 × 360 ml, Lysis buffer type 12: 2 × 360 ml, Lysis buffer type 13: 2 × 360 ml, Wash buffer type 2: 5 × 480 ml, Elution buffer type 3: 480 ml, TE buffer type 1: 120 ml, illustra Fast-Flow plasmid 250 columns: 2 × 50, Column stand: 1, Waste reservoir: 1, Adapters: 12