Cytiva's Whatman™ 38152103011150 Sera-Mag™ Sera-Mag Oligo (dT) Coated Magnetic Particles, 1mL, Storage Conditions: 4°C, Average Particle Size: 1µm

  • Binding Capacity: > 300 pmol permg of particle
  • Ligand: Oligo (dT), Storage conditions: 4°C
  • Concentration: 10mg/ml, Average Particle Size: 1µm
  • Magnetite Content: ~40%, Covalently bound oligo(dT)14 prevents leaching from the particle surface
  • Fast reaction kinetics increases throughput and precision, and also enables faster movement through viscous solutions
  • Sold Individually
  • Manufacturer Part No: WHA-38152103011150
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Item Number: WHA-38152103011150
$176.82

Sold in: Eaches

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Sera-Mag Oligo(dT)-Coated Magnetic Particles enable convenient mRNA isolation and extraction. Convenient isolation and extraction of mRNA from a variety of sources for downstream applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization. Covalently bound oligo(dT)14 prevents leaching from the particle surface. Very high, specific poly A+ binding capacity ensures maximum extraction of mRNA. Fast reaction kinetics increases throughput and precision, and also enables faster movement through viscous solutions. Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications. Uniform, nominal 1 μm diameter provides high surface area and excellent lot-to-lot reproducibility. Convenient and Efficient mRNA Isolation Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions. Versatile and efficient methods for mRNA purification are critical, given that eukaryotic mRNA makes up only 1 - 3% of total cellular RNA. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. Fast Magnetic Response Times and Stable Physical Integrity The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalenty bound to the particle surface

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Sera-Mag Oligo(dT)-Coated Magnetic Particles enable convenient mRNA isolation and extraction. Convenient isolation and extraction of mRNA from a variety of sources for downstream applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension, and subtractive hybridization. Covalently bound oligo(dT)14 prevents leaching from the particle surface. Very high, specific poly A+ binding capacity ensures maximum extraction of mRNA. Fast reaction kinetics increases throughput and precision, and also enables faster movement through viscous solutions. Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications. Uniform, nominal 1 μm diameter provides high surface area and excellent lot-to-lot reproducibility. Convenient and Efficient mRNA Isolation Sera-Mag Oligo(dT) Magnetic Particles bind target mRNA through pairing of the polyadenylated RNA tail found on the 3’ end of mRNA to the covalently bound oligo(dT) groups on the surface of the particles. This binding is easily accomplished using standard hybridization conditions. Versatile and efficient methods for mRNA purification are critical, given that eukaryotic mRNA makes up only 1 - 3% of total cellular RNA. Sera-Mag Oligo(dT) Magnetic Particles remove 90% or more of mRNA from total RNA with just one extraction. Fast Magnetic Response Times and Stable Physical Integrity The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One layer of magnetite is coated onto this core, while the surface is modified to minimize non-specific binding of proteins. Oligo(dT) groups are then covalenty bound to the particle surface

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