Cytiva's Whatman™ 80-6484-70 SDS-Page Clean-Up Kit
- Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or low protein concentrations
- Quantitatively precipitate proteins while leaving interfering substances in solution
- Max Sample Volume: 100μl
- Sold Individually
- Manufacturer Part No: WHA-80-6484-70
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Details
Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or low protein concentrations Quantitatively precipitate proteins while leaving interfering substances in solution Delivers quantitative yield in less than 2 h. SDS-PAGE Clean-Up Kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the proteins are pelleted by centrifugation. The precipitate is washed further to remove nonprotein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 h with quantitative yield The kit should be stored at room temperature. The wash buffer should be placed at -20°C at least 1 h before use and may be stored in a -20°C freezer15 ml Precipitant 15 ml Coprecipitant 50 ml Wash buffer 250μl Wash additive 2 ml Buffer I 500 μl Buffer II Quick reference card Instructions
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Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or low protein concentrations Quantitatively precipitate proteins while leaving interfering substances in solution Delivers quantitative yield in less than 2 h. SDS-PAGE Clean-Up Kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the proteins are pelleted by centrifugation. The precipitate is washed further to remove nonprotein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 h with quantitative yield The kit should be stored at room temperature. The wash buffer should be placed at -20°C at least 1 h before use and may be stored in a -20°C freezer15 ml Precipitant 15 ml Coprecipitant 50 ml Wash buffer 250μl Wash additive 2 ml Buffer I 500 μl Buffer II Quick reference card Instructions