Cytiva's Whatman™ RPN2108 Amersham™ ECL Western Blotting Detection Kit
- Sold Individually
- Manufacturer Part No: WHA-RPN2108
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Details
Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane. Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents. Optimized system provides rapid, sensitive results using simple protocols. Designed for use with nitrocellulose and PVDF membranes. High sensitivity: Up to 10 times more sensitive than colorimetric methods. Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods. Easy reprobing: Save time and materials by reprobing on membranes up to 10 times. Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL, Non-radioactive-no specialized facilities needed Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide Amersham™ ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera
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Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane. Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents. Optimized system provides rapid, sensitive results using simple protocols. Designed for use with nitrocellulose and PVDF membranes. High sensitivity: Up to 10 times more sensitive than colorimetric methods. Rapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods. Easy reprobing: Save time and materials by reprobing on membranes up to 10 times. Quantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL, Non-radioactive-no specialized facilities needed Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide Amersham™ ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera