QIAGEN® GBS WB129243 FTA™ DMPK-C Cards for Dried Blood Spot (DBS) DNA Analysis & Archiving (Pack of 100)

Cutting the Cost of Generating PK/TK Data: Pharmacokinetic (PK) and toxicokinetic (TK) studies provide crucial insight into how drug candidates behave in the body. They are therefore a critical step in drug development. These analyses traditionally require large volumes of blood (generally 100–500?L per subject per time-point) in order to provide sufficient plasma volume for quantitative bioanalysis. Additionally, the plasma must be isolated from whole blood and prepared for bioanalysis using solid phase extraction, liquid-liquid extraction, or protein precipitation. This time consuming process limits throughput and consequently the number of samples that can be tested. Finally, there are the practical challenges of shipping and storing blood samples, which require controlled handling and frozen transportation and storage. The Dried Blood Spot (DBS) method is an alternative technique that overcomes these drawbacks and delivers significant cost savings. Dried Blood Spot (DBS) micro-volume sampling using specialized Cytiva Whatman™ media has been shown to be precise for a variety of compounds from different structural classes with acceptable inter- and intra-assay variability and is routinely employed in PK/TK studies. The FTA™ DMPK-C cards have no impregnated chemicals to interfere with analysis. Proteins are not denatured so cards may be better suited for protein based biomolecules. Samples can be shipped and stored at ambient temperature and long-term stability has been demonstrated for analytes and metabolites sensitive to plasma enzymes. Low blood volumes are required for FTA™ DMPK-C cards in amounts of only 10 to 20?L per sample. This means that fewer experimental animals are required because toxicokinetic data is derived from toxicology animals, thereby eliminating satellite colonies. More consistent data is obtained through more serial sampling from individual animals and less reliance is required on composite data, further reducing the number of animals needed. Juvenile animals and humans can also be studied. The (DBS) Dried Blood Spot Procedure (Spot. Extract. Analyze.): (1) Apply blood to card and allow to dry. Ship and store as needed at ambient temperature. (2) Remove sample disc and elute with solvent. (3) Analyze solution by HPLC-MS/MS. FTA™ DMPK-A Cards provide simple and safe processing. The 3-step DBS procedure is much more straightforward than the cumbersome centrifugation, isolation and clean-up of plasma. On-substrate clean-up has the potential to convey greater analyte stability, especially for enzyme-sensitive compounds. Room temperature stability saves on cost of dry ice shipments which makes this method simpler and more cost effective to carry out remote sampling or clinical studies. Getting Started: First-time trial and use of QIAGEN® FTA™ DMPK cards, using the Dried Blood Spot method, the choice of DMPK card depends on many factors including analyte chemical structure, extraction solvent and analysis workflow. The final decision is usually determined empirically. The QIAGEN® FTA™ DMPK Card Starter Pack Kit (WB129428) is ideal for first-time use and evaluation. Starter pack kits include fifteen cards of FTA™ DMPK-A, DMPK-B and DMPK-C respectively along with a 3.0mm Uni-Core Punch punches and a small cutting mat. Once the right FTA™ DMPK card is determined, that specific card can be ordered individually. Contact Cytiva's expert DMPK Team for specific advice on card selection and your specific methods. FTA™ DMPK-C Card Features: (1) Blood spots dry within 2-Hours. (2) Blood spot area is ~20% larger than DMPK-A cards (3) No impregnated chemicals to interfere with analysis. (4) Proteins are not denatured so cards may be better suited for protein based biomolecules. 100 Cards/Pack.

Cutting the Cost of Generating PK/TK Data: Pharmacokinetic (PK) and toxicokinetic (TK) studies provide crucial insight into how drug candidates behave in the body. They are therefore a critical step in drug development. These analyses traditionally require large volumes of blood (generally 100–500?L per subject per time-point) in order to provide sufficient plasma volume for quantitative bioanalysis. Additionally, the plasma must be isolated from whole blood and prepared for bioanalysis using solid phase extraction, liquid-liquid extraction, or protein precipitation. This time consuming process limits throughput and consequently the number of samples that can be tested. Finally, there are the practical challenges of shipping and storing blood samples, which require controlled handling and frozen transportation and storage. The Dried Blood Spot (DBS) method is an alternative technique that overcomes these drawbacks and delivers significant cost savings. Dried Blood Spot (DBS) micro-volume sampling using specialized Cytiva Whatman™ media has been shown to be precise for a variety of compounds from different structural classes with acceptable inter- and intra-assay variability and is routinely employed in PK/TK studies. The FTA™ DMPK-C cards have no impregnated chemicals to interfere with analysis. Proteins are not denatured so cards may be better suited for protein based biomolecules. Samples can be shipped and stored at ambient temperature and long-term stability has been demonstrated for analytes and metabolites sensitive to plasma enzymes. Low blood volumes are required for FTA™ DMPK-C cards in amounts of only 10 to 20?L per sample. This means that fewer experimental animals are required because toxicokinetic data is derived from toxicology animals, thereby eliminating satellite colonies. More consistent data is obtained through more serial sampling from individual animals and less reliance is required on composite data, further reducing the number of animals needed. Juvenile animals and humans can also be studied. The (DBS) Dried Blood Spot Procedure (Spot. Extract. Analyze.): (1) Apply blood to card and allow to dry. Ship and store as needed at ambient temperature. (2) Remove sample disc and elute with solvent. (3) Analyze solution by HPLC-MS/MS. FTA™ DMPK-A Cards provide simple and safe processing. The 3-step DBS procedure is much more straightforward than the cumbersome centrifugation, isolation and clean-up of plasma. On-substrate clean-up has the potential to convey greater analyte stability, especially for enzyme-sensitive compounds. Room temperature stability saves on cost of dry ice shipments which makes this method simpler and more cost effective to carry out remote sampling or clinical studies. Getting Started: First-time trial and use of QIAGEN® FTA™ DMPK cards, using the Dried Blood Spot method, the choice of DMPK card depends on many factors including analyte chemical structure, extraction solvent and analysis workflow. The final decision is usually determined empirically. The QIAGEN® FTA™ DMPK Card Starter Pack Kit (WB129428) is ideal for first-time use and evaluation. Starter pack kits include fifteen cards of FTA™ DMPK-A, DMPK-B and DMPK-C respectively along with a 3.0mm Uni-Core Punch punches and a small cutting mat. Once the right FTA™ DMPK card is determined, that specific card can be ordered individually. Contact Cytiva's expert DMPK Team for specific advice on card selection and your specific methods. FTA™ DMPK-C Card Features: (1) Blood spots dry within 2-Hours. (2) Blood spot area is ~20% larger than DMPK-A cards (3) No impregnated chemicals to interfere with analysis. (4) Proteins are not denatured so cards may be better suited for protein based biomolecules. 100 Cards/Pack.