Whatman™ Protran™ Nicrocellulose Blotting Membranes

Whatman™ Protran™ blotting membrane filters are available in 0.1, 0.2 or 0.45µm pore sizes and are the ideal membranes for capturing proteins. Protran™ blotting membranes have the best handling strength of all pure nitrocellulose membranes. They are compatible with a variety of detection methods, including isotopic, chemiluminescent (luminol-based), colorimetric, and fluorescent. Unlike PVDF membranes, Protran™ nitrocellulose does not require a methanol pre-wetting step. This makes Protran™ the membrane of choice for proteins which prefer aqueous environments. Prior to transfer, the membrane is simply wetted in water, and then placed in the transfer buffer. No other pretreatment steps are necessary. High binding, low background: In addition to high binding capacity, Protran™ nitrocellulose blotting membranes inherently have very low background. The superior surface properties of the membrane guarantee superior signal-to-noise ratios, without the need for stringent washing conditions.

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