Axygen® Nucleic Acid Purification Kits, Corning®

Axygen® AxyPrep™ Mag Dye Cleanup Kits

The AxyPrep™ Mag dye cleanup kit is simple and easy sequencing reaction clean up process, takes about 25 min/96 samples. Excess dye terminator clean up post sequencing reaction clean up is an essential step prior to sequencing. The carry over of excess dye into sequencing results in dye blobs which leads to overwhelming noise and failed calls of sequenced bases and potentially leading to the failure the sequencing run. AxyPrep Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean up of excess dye terminator post sequencing reaction in Sanger sequencing. The protocol mainly consists of binding, washing and elution steps, and can be performed directly in the thermal cycling plate and requires no centrifugation or filtration.