Axygen® AxyPrep™ Plasmid DNA Purification Maxiprep Kits, Corning®
This AxyPrep™ Plasmid Maxiprep kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for kit is up to 500µg respectively. This kit can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified plasmid DNA. The entire preparation process can be completed in 45 minutes for the Maxiprep. Plasmid prepared by this method can be used immediately for many routine applications requiring highly purified plasmid DNA, such as, DNA sequencing, restriction digestion, in vitro transcription, library screening, ligation and bacterial transformation.