The proprietary Whatman™ UNIFILTER® microplates with filter-bottom wells are convenient and ready to use. Available in 24 well, 96 well and 384 well microplate formats. Whatman UNIFILTER microplates offer a choice of filter media to meet exact application requirements. The novel drip director design of Whatman UNIFILTER microplates ensures precise collection of the filtrate to allow for further processing and analysis. UNIFILTER microplates are available in a range of well volumes from 100µl to 10mL. Features & Benefits: No cross talk. Patented integral filter design prevents well-to-well cross contamination. Economical to use. Wide range of well volume options ensures efficient use of materials. Better control. Choice of filter media allows control of the flow rates and retention characteristics. A broad range of filtration media is available including glass fiber, polypropylene, cellulose nitrate, cellulose acetate, nylon and ion exchange cellulose.
The Whatman 24-Well x 10mL UNIFILTER® filter bottom microplate is widely used for applications that require very large sample or reagent volumes. Typically these applications include biomolecule purification by solid phase extraction and organic synthesis in combinatorial chemistry library generation. The polypropylene construction of this 10mL UNIFILTER® filter bottom microplate enables chemical and heat-resistant operation. The patented integral design of the Long Drip Directors facilitates collection of filtrate with no cross talk.
The Whatman™ 800µL UNIFILTER® is the filtering microplate most typically used in purifications, isolations, and separations of biomolecules - particularly in DNA purification. These filtering microplates have a well volume of 800µL which is the ideal size for standard DNA plasmid miniprep chemistries. Whatman™ UNIFILTER 800µL filtering microplates are manufactured from clear, rigid high-grade polystyrene and feature integral short drip directors which facilitate collection of filtrate with no cross talk.
The 96-Well x 2mL UNIFILTER® filtering microplate is widely used for applications that require larger sample or reagent volumes. Typically these applications include biomolecule purification by solid phase extraction and organic synthesis in combinatorial chemistry library generation. The glass-filled polypropylene construction of the 2mL UNIFILTER® filter bottom microplate enables chemical and heat-resistant operation. The long drip directors facilitate collection of filtrate with no cross talk. Chemically resistant UNIFILTER® filtering microplates are furnished with a variety of choice in membrane filters.
The Whatman™ UNIFILTER® FF Protein Precipitation (Fast Flow) filtering microplate is optimized for removing acetonitrile-precipitated proteins from plasma or serum samples. This 2mL x 96-Well, rigid glass-filled polypropylene microplate is both robust and chemically resistant. The microplate contains specially formulated dual membranes with two distinct layers. The top layer acts as a prefilter to remove coarse particulates. The bottom layer is oleophobic for retaining the well contents without dripping. This provides a final filter for removing fine particulate matter when a vacuum is applied. Purified & process samples are ready in less than ten minutes with precipitation and filtration occurring in the same well. These filtering microplates eliminated the need for laborious centrifugation or pipetting with minimal liquid handling. Microplates deliver 98% protein removal and are ideal for sample volumes of up to 150µL for plasma and 200µL for serum. ANSI/SBS compliant and automation/robotics-friendly.
The 100µL UNIFILTER® is the only 384-Well filtering microplate with a 100µL well volume to allow a large enough sample for recovery after filtration. The 384-Well filter bottom microplate includes Whatman’s integral, patented Long-Drip Director design which eliminates well-to-well contamination during the filtration process. The 384-Well filter bottom microplate is ideal for DNA template clean-up, cell capture, and for the removal of unwanted debris.
Whatman™ has developed consistent and reproducible microplates and microplate systems to improve throughput and reduce cost for a number of biological sample preparations and clean-up procedures.
Comprehensive protocols are provided to enable implementation by all types of users. Whatman microplates conform to the ANSI/SBS standards and are engineered for fast and convenient processing applications.