MilliporeSigma VSWP02500 MF-Millipore™ DNA Fillter Paper for Dialysis of DNA and Proteins, Plain White Mixed Cellulose Esters, 0.025µm, Diameter: 2.5m (Pack of 100)

Millipore MF-Millipore™ VSWP plain white mixed cellulose ester membrane filters are designed for microdialysis (desalting) of DNA and proteins. These mixed cellulose esters membrane discs are a convenient, high recovery way to dialyze micro-volumes of protein or nucleic acid solutions (<100µL). With the “drop dialysis” technique, a floating filter disc replaces the dialysis sac. Recoveries of DNA and protein samples have been reported in excess of 98% (Marusyk, R. and Sergeant, A., “A Simple Method for Dialysis of Small-Volume Samples,” Analytical Biochem. 105, pp. 403–404, 1980). Drop Dialysis Technique: 1. Fill the bottom of a Petri dish with buffer solution. 2. Float the membrane filter disc on the surface of the buffered solution. 3. Deposit 5 to 100µL of sample on the center of the membrane. Place a tight-fitting lid on the Petri dish to prevent evaporation. Marker dyes may help in determining the dialysis time required. Most samples are dialyzed in less than 30-minutes. 4. Recover the desalted sample. Filter material: Mixed Cellulose Esters. Filter Color: White. Filter Surface: Plain (non-gridded)

Millipore MF-Millipore™ VSWP plain white mixed cellulose ester membrane filters are designed for microdialysis (desalting) of DNA and proteins. These mixed cellulose esters membrane discs are a convenient, high recovery way to dialyze micro-volumes of protein or nucleic acid solutions (<100µL). With the “drop dialysis” technique, a floating filter disc replaces the dialysis sac. Recoveries of DNA and protein samples have been reported in excess of 98% (Marusyk, R. and Sergeant, A., “A Simple Method for Dialysis of Small-Volume Samples,” Analytical Biochem. 105, pp. 403–404, 1980). Drop Dialysis Technique: 1. Fill the bottom of a Petri dish with buffer solution. 2. Float the membrane filter disc on the surface of the buffered solution. 3. Deposit 5 to 100µL of sample on the center of the membrane. Place a tight-fitting lid on the Petri dish to prevent evaporation. Marker dyes may help in determining the dialysis time required. Most samples are dialyzed in less than 30-minutes. 4. Recover the desalted sample. Filter material: Mixed Cellulose Esters. Filter Color: White. Filter Surface: Plain (non-gridded)