Axygen® AxyPrep™ Mag Tissue Genomic DNA Purification Kits, Corning®
The AxyPrep™ Mag tissue genomic DNA kit utilizes a magnetic bead based technology to isolate genomic DNA from rodent tail tissues. This protocol provides instructions to extract DNA from fresh or frozen rodent tails. The protocol is performed in 96-well format and 1.5mL. Purification begins by the addition of lysis buffer, and Proteinase K to rupture cell membranes and digest protein. DNA is then immobilized on magnetic particles by the addition of a magnetic binding reagent. This differential binding allows the DNA to be easily separated from contaminants using a magnetic field. Contaminants can then be rinsed away using a simple washing procedure.